Hotspot

trnV-GAC-rps12

IRa · 127066-128772 · 1707 bp

Back to Setaria

Pi 0.00186
MarkerSeek score 59.90
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1707
variable_sites
6
pi
0.00186
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA CGTAAGGCAGAGTTGGGTTT 50.0 50.0 57.8 58.1 1792-1820 bp 1.0 78.3
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG CGTAAGGCAGAGTTGGGTTT 50.0 50.0 57.8 58.1 1796-1824 bp 1.0 78.3
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG CGTAAGGCAGAGTTGGGTTT 50.0 50.0 57.8 58.1 1791-1819 bp 1.0 78.3
trnV-GAC-rps12_p4 TGGTGGGGGAGTAAGTCAAA CGTAAGGCAGAGTTGGGTTT 50.0 50.0 58.2 58.1 1851-1879 bp 1.0 78.2
trnV-GAC-rps12_p5 CTGATGACTTCCACCACGTC CGTAAGGCAGAGTTGGGTTT 55.0 50.0 58.3 58.1 1794-1822 bp 1.0 77.3