Hotspot

rpl32-trnL-UAG

SSC · 107571-107960 · 390 bp

Back to Setaria

Pi 0.012

MarkerSeek score 51.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 390
variable_sites: 9
pi: 0.012
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAGGATTGATTTGCGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	675-704 bp	1.0	80.5
rpl32-trnL-UAG_p2	AGAAGGATTGATTTGCGGCA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.2	58.0	1254-1283 bp	1.0	80.2
rpl32-trnL-UAG_p3	AGAAGGATTGATTTGCGGCA	ATGTCCCGAAGAAACCCAAC	45.0	50.0	58.2	58.1	988-1017 bp	1.0	80.1
rpl32-trnL-UAG_p4	AGAAGGATTGATTTGCGGCA	CCGAAGAAACCCAACGAGAA	45.0	50.0	58.2	58.1	983-1012 bp	1.0	79.9
rpl32-trnL-UAG_p5	AGAAGGATTGATTTGCGGCA	AATGATTAGAAGAGCGGCGG	45.0	50.0	58.2	58.1	1282-1311 bp	1.0	79.3