Hotspot

rps12-trnV-GAC

IRb · 92412-94118 · 1707 bp

Back to Setaria

Pi 0.00186

MarkerSeek score 60.00

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1707
variable_sites: 6
pi: 0.00186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	CGTAAGGCAGAGTTGGGTTT	GATGACTTCCACCACGTCAA	50.0	50.0	58.1	57.8	1791-1820 bp	1.0	78.4
rps12-trnV-GAC_p2	CGTAAGGCAGAGTTGGGTTT	AACTGATGACTTCCACCACG	50.0	50.0	58.1	57.8	1795-1824 bp	1.0	78.3
rps12-trnV-GAC_p3	CGTAAGGCAGAGTTGGGTTT	ATGACTTCCACCACGTCAAG	50.0	50.0	58.1	57.8	1790-1819 bp	1.0	78.3
rps12-trnV-GAC_p4	CGTAAGGCAGAGTTGGGTTT	TGGTGGGGGAGTAAGTCAAA	50.0	50.0	58.1	58.2	1850-1879 bp	1.0	78.2
rps12-trnV-GAC_p5	CGTAAGGCAGAGTTGGGTTT	CTGATGACTTCCACCACGTC	50.0	55.0	58.1	58.3	1793-1822 bp	1.0	77.4