Hotspot

psbM-petN

LSC · 18359-19159 · 801 bp

Back to Setaria

Pi 0.0132

MarkerSeek score 70.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 18
pi: 0.0132
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	825-908 bp	1.0	74.0
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	56.7	58.3	769-852 bp	1.0	73.1
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	56.7	58.4	768-851 bp	1.0	72.4
psbM-petN_p4	AAACAGCCAGCCAAAATGAT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	824-907 bp	1.0	72.2
psbM-petN_p5	AACAGCCAGCCAAAATGATT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	823-906 bp	1.0	72.2