Hotspot

trnD-GUC-psbM

LSC · 17208-18253 · 1046 bp

Back to Setaria

Pi 0.0121
MarkerSeek score 61.60
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1046
variable_sites
25
pi
0.0121
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTT 50.0 40.0 58.5 56.7 1199-1233 bp 1.0 68.7
trnD-GUC-psbM_p2 TAGGTATGCCATACACCCCG TCATTTTGGCTGGCTGTTTT 55.0 40.0 58.7 56.7 1231-1265 bp 1.0 67.2
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG AATCATTTTGGCTGGCTGTT 50.0 40.0 58.5 56.5 1201-1235 bp 1.0 66.8
trnD-GUC-psbM_p4 TTCAATTGGTCAGAGCACCG ATCATTTTGGCTGGCTGTTT 50.0 40.0 58.5 56.5 1200-1234 bp 1.0 66.8
trnD-GUC-psbM_p5 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTTT 50.0 38.1 58.5 57.4 1199-1233 bp 1.0 66.6