Hotspot

trnG-UCC-trnM-CAU

LSC · 14020-15959 · 1940 bp

Back to Setaria

Pi 0.0126
MarkerSeek score 60.40
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1940
variable_sites
43
pi
0.0126
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1832-2041 bp 1.0 46.5
trnG-UCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1828-2037 bp 1.0 45.8
trnG-UCC-trnM-CAU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1829-2038 bp 1.0 45.8
trnG-UCC-trnM-CAU_p4 AGAACGAATCACACTTTTACCAC TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1835-2044 bp 1.0 45.8
trnG-UCC-trnM-CAU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1834-2043 bp 1.0 45.8