Hotspot

trnK-UUU-rps16

LSC · 3922-4464 · 543 bp

Back to Setaria

Pi 0.0145

MarkerSeek score 55.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 543
variable_sites: 14
pi: 0.0145
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TGAGGATCAGTCGTGGTCTT	ATTTCCTTGAAACGGGTGCT	50.0	45.0	58.1	58.0	760-773 bp	1.0	82.2
trnK-UUU-rps16_p2	AGGATCAGTCGTGGTCTTCT	ATTTCCTTGAAACGGGTGCT	50.0	45.0	57.8	58.0	758-771 bp	1.0	81.0
trnK-UUU-rps16_p3	TGAGGATCAGTCGTGGTCTT	ATACGTTTCTAGGGGAGGGG	50.0	55.0	58.1	57.9	927-940 bp	1.0	80.9
trnK-UUU-rps16_p4	TGAGGATCAGTCGTGGTCTT	TTCTAGGGGAGGGGTTGTTT	50.0	50.0	58.1	57.9	921-934 bp	1.0	80.6
trnK-UUU-rps16_p5	TGAGGATCAGTCGTGGTCTT	TTTCTAGGGGAGGGGTTGTT	50.0	50.0	58.1	57.9	922-935 bp	1.0	80.6