Hotspot

ndhD

SSC · 117677-119179 · 1503 bp

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Pi 0.012

MarkerSeek score 51.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 18
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAGTGCTTCCCCCTTTTCTG	TCTGCTTGCCCAACTGATTT	50.0	45.0	58.0	58.0	2282-2288 bp	1.0	81.8
ndhD_p2	ATGCAGCTCTTTTATGCGGA	TCTGCTTGCCCAACTGATTT	45.0	45.0	58.0	58.0	2448-2454 bp	1.0	81.5
ndhD_p3	TGCAGCTCTTTTATGCGGAT	TCTGCTTGCCCAACTGATTT	45.0	45.0	58.0	58.0	2447-2453 bp	1.0	81.5
ndhD_p4	GAGCAGTATGGGCCAATGAA	TCTGCTTGCCCAACTGATTT	50.0	45.0	57.9	58.0	2160-2166 bp	1.0	81.4
ndhD_p5	AGCAGTATGGGCCAATGAAG	TCTGCTTGCCCAACTGATTT	50.0	45.0	57.9	58.0	2159-2165 bp	1.0	81.3