Hotspot

rpl32-trnL-UAG

SSC · 115044-116276 · 1233 bp

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Pi 0.0272
MarkerSeek score 60.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1233
variable_sites
33
pi
0.0272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGTTGGAAAGACAAGGGAT ACTGGGATTGTAGAGCAGGT 45.0 50.0 56.6 58.0 1972-1975 bp 1.0 71.9
rpl32-trnL-UAG_p2 ATGGTTGGAAAGACAAGGGA AGCGTGTCTACCGATTTCAC 45.0 50.0 56.6 58.0 1378 bp 0.5 49.0
rpl32-trnL-UAG_p3 TGGTTGGAAAGACAAGGGAT AGCGTGTCTACCGATTTCAC 45.0 50.0 56.6 58.0 1377 bp 0.5 49.0
rpl32-trnL-UAG_p4 ATGGTTGGAAAGACAAGGGA CAGCGTGTCTACCGATTTCA 45.0 50.0 56.6 58.0 1379 bp 0.5 49.0
rpl32-trnL-UAG_p5 TGGTTGGAAAGACAAGGGAT CAGCGTGTCTACCGATTTCA 45.0 50.0 56.6 58.0 1378 bp 0.5 49.0