Hotspot

psbT-psbN

LSC · 75797-75856 · 60 bp

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Pi 0.1

MarkerSeek score 74.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 6
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCCCCTAAAGTTCCAACTCC	CGAGGAACACGGGGACTA	55.0	61.1	57.5	58.0	118 bp	1.0	63.9
psbT-psbN_p2	CCCCCTAAAGTTCCAACTCC	CGAGGAACACGGGGACTAG	55.0	63.2	57.5	59.2	118 bp	1.0	62.5
psbT-psbN_p3	CCCCCTAAAGTTCCAACTCC	TTCGAGGAACACGGGGACTA	55.0	55.0	57.5	60.3	120 bp	1.0	61.9
psbT-psbN_p4	CCCCCTAAAGTTCCAACTCC	TCGAGGAACACGGGGACTA	55.0	57.9	57.5	59.6	119 bp	1.0	59.0
psbT-psbN_p5	CCCCCTAAAGTTCCAACTCC	TCGAGGAACACGGGGACTAG	55.0	60.0	57.5	60.7	119 bp	1.0	58.5