Hotspot

rpl20-rps12

LSC · 70296-71098 · 803 bp

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Pi 0.0118

MarkerSeek score 57.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 9
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTATTCTACGTCTCCGGGCT	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	837-876 bp	1.0	83.0
rpl20-rps12_p2	CCCTCGAAAGCTTGATCCAA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	869-908 bp	1.0	82.4
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	880-919 bp	1.0	82.4
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	879-918 bp	1.0	82.4
rpl20-rps12_p5	ATTCTACGTCTCCGGGCTAT	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	835-874 bp	1.0	81.2