Hotspot

psbE-petL

LSC · 65857-67001 · 1145 bp

Back to Sesuvium

Pi 0.0273

MarkerSeek score 69.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 31
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.9	56.8	1338-1366 bp	1.0	72.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCT	50.0	50.0	57.9	56.8	1337-1365 bp	1.0	72.0
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGCTCAGACCT	50.0	47.6	57.9	58.3	1338-1366 bp	1.0	71.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	1261-1289 bp	1.0	71.3
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCT	50.0	50.0	57.8	56.8	1260-1288 bp	1.0	71.3