Hotspot

petA-psbJ

LSC · 64139-65068 · 930 bp

Back to Sesuvium

Pi 0.0216

MarkerSeek score 71.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 930
variable_sites: 20
pi: 0.0216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATTGCGCGTACAAGGTCTTT	ATTCTGGATTGGGCTCATCC	45.0	50.0	58.5	57.3	1071-1080 bp	1.0	72.6
petA-psbJ_p2	CGAGAAGGTTCAACTGTCCG	ATTCTGGATTGGGCTCATCC	55.0	50.0	58.6	57.3	990-999 bp	1.0	71.9
petA-psbJ_p3	TTGCGCGTACAAGGTCTTTT	ATTCTGGATTGGGCTCATCC	45.0	50.0	58.7	57.3	1070-1079 bp	1.0	70.8
petA-psbJ_p4	GAGAAGGTTCAACTGTCCGA	ATTCTGGATTGGGCTCATCC	50.0	50.0	57.2	57.3	989-998 bp	1.0	70.0
petA-psbJ_p5	TCGAGAAGGTTCAACTGTCC	ATTCTGGATTGGGCTCATCC	50.0	50.0	57.2	57.3	991-1000 bp	1.0	70.0