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atpH-atpI

LSC · 14173-14893 · 721 bp

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Pi 0.0185

MarkerSeek score 71.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 13
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGTACCCTGACCAACTCCAG	ACAAGTGGTATCCAGGCTCT	55.0	50.0	58.4	58.0	889-893 bp	1.0	79.5
atpH-atpI_p2	AGGTCCAATAGAAGCAAGCC	ACAAGTGGTATCCAGGCTCT	50.0	50.0	57.6	58.0	871-875 bp	1.0	79.0
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCT	ACAAGTGGTATCCAGGCTCT	50.0	50.0	57.6	58.0	869-873 bp	1.0	79.0
atpH-atpI_p4	AGTACCCTGACCAACTCCAG	TTTTGCAACTTTAGCTGCCG	55.0	45.0	58.4	57.9	866-870 bp	1.0	78.9
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCT	TTTTGCAACTTTAGCTGCCG	50.0	45.0	57.6	57.9	846-850 bp	1.0	78.4