Hotspot

trnK-UUU-rps16

LSC · 4421-5284 · 864 bp

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Pi 0.034
MarkerSeek score 68.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
864
variable_sites
27
pi
0.034
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1638-1671 bp 1.0 84.9
trnK-UUU-rps16_p2 CGAATCCCTTGCTTCATCCA AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1591-1624 bp 1.0 84.8
trnK-UUU-rps16_p3 ACGAATCCCTTGCTTCATCC AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1592-1625 bp 1.0 84.8
trnK-UUU-rps16_p4 TCAGGATCAGTCGTGGTCTT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.1 58.0 1634-1667 bp 1.0 84.4
trnK-UUU-rps16_p5 TTCAGGATCAGTCGTGGTCT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.1 58.0 1635-1668 bp 1.0 84.4