Hotspot

rpoA-rps11

LSC · 75260-75323 · 64 bp

Back to Sesleria

Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	GGGACAGAATGAAACGACCA	CCATTGCTAAAAGCGGTGTG	50.0	50.0	57.8	58.0	265 bp	1.0	78.7
rpoA-rps11_p2	GGGACAGAATGAAACGACCA	GTGGAAGAGATGCCGCATTA	50.0	50.0	57.8	58.0	289 bp	1.0	78.5
rpoA-rps11_p3	GGGACAGAATGAAACGACCA	TGGAAGAGATGCCGCATTAC	50.0	50.0	57.8	58.0	288 bp	1.0	78.5
rpoA-rps11_p4	GGGACAGAATGAAACGACCA	GATGTAACACCTATGCCGCA	50.0	50.0	57.8	58.1	227 bp	1.0	78.5
rpoA-rps11_p5	GGGACAGAATGAAACGACCA	ACACCTATGCCGCATAATGG	50.0	50.0	57.8	58.1	221 bp	1.0	78.1