Hotspot

rpl20-rps12

LSC · 66632-67327 · 696 bp

Back to Sesleria

Pi 0.00575

MarkerSeek score 61.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 4
pi: 0.00575
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	45.0	55.0	58.0	58.0	773 bp	1.0	80.1
rpl20-rps12_p2	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	45.0	55.0	58.0	58.1	776 bp	1.0	79.7
rpl20-rps12_p3	GTTTGAGGCAAATGAACGCA	GAGGAACATGTGCTAGGGTG	45.0	55.0	57.9	58.0	788 bp	1.0	79.1
rpl20-rps12_p4	GTTTGAGGCAAATGAACGCA	GTCGAGGAACATGTGCTAGG	45.0	55.0	57.9	58.1	791 bp	1.0	78.8
rpl20-rps12_p5	TTTTTGTTCGTCGTCTCCGA	GAGGAACATGTGCTAGGGTG	45.0	55.0	57.8	58.0	768 bp	1.0	78.5