Hotspot

ycf3-trnS-GGA

LSC · 43805-44426 · 622 bp

Back to Sesleria

Pi 0.00482

MarkerSeek score 61.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 3
pi: 0.00482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	805 bp	1.0	78.6
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTCAA	50.0	45.0	58.1	57.8	755 bp	1.0	78.3
ycf3-trnS-GGA_p3	TCTCCTGAGGTTGTCGGAAT	AGTTCCAATGCTACGCCTTC	50.0	50.0	58.1	58.3	757 bp	1.0	77.7
ycf3-trnS-GGA_p4	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	806 bp	1.0	76.8
ycf3-trnS-GGA_p5	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	807 bp	1.0	76.8