Hotspot

trnG-GCC-trnfM-CAU

LSC · 12217-12714 · 498 bp

Back to Sesleria

Pi 0.00602
MarkerSeek score 52.10
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
498
variable_sites
3
pi
0.00602
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 GTCGACCCAAGCGGATATAC TTTTTGGTACTCCAGAGCGG 55.0 50.0 57.9 57.8 698-726 bp 1.0 78.4
trnG-GCC-trnfM-CAU_p2 GCGAGCGTAGTTCAATGGTA TTTTTGGTACTCCAGAGCGG 50.0 50.0 58.1 57.8 658-686 bp 1.0 78.3
trnG-GCC-trnfM-CAU_p3 GTCGACCCAAGCGGATATAC CGAGGCTCATAACCTTGAGG 55.0 55.0 57.9 57.8 656-684 bp 1.0 78.0
trnG-GCC-trnfM-CAU_p4 GCGAGCGTAGTTCAATGGTA CGAGGCTCATAACCTTGAGG 50.0 55.0 58.1 57.8 616-644 bp 1.0 77.9
trnG-GCC-trnfM-CAU_p5 GTCGACCCAAGCGGATATAC CAGTTTGGTAGCTCACGAGG 55.0 55.0 57.9 58.3 671-699 bp 1.0 77.6