Hotspot

psbI-trnS-GCU

LSC · 7450-7568 · 119 bp

Back to Sesleria

Pi 0.0087

MarkerSeek score 60.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 1
pi: 0.0087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TCTAATGACCCAGGACGGAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.8	58.0	222-226 bp	1.0	79.5
psbI-trnS-GCU_p2	ATCTAATGACCCAGGACGGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.5	58.0	223-227 bp	1.0	77.7
psbI-trnS-GCU_p3	TCTAATGACCCAGGACGGAA	TGATTTGGAACGGGGAGAGA	50.0	50.0	57.8	58.3	255-259 bp	1.0	76.8
psbI-trnS-GCU_p4	TCTAATGACCCAGGACGGAA	ATGGCTGAGTGGACTAAAGC	50.0	50.0	57.8	57.6	236-240 bp	1.0	76.5
psbI-trnS-GCU_p5	TCTAATGACCCAGGACGGAA	TTGATTTGGAACGGGGAGAG	50.0	50.0	57.8	57.5	256-260 bp	1.0	75.7