Hotspot

rps16-trnQ-UUG

LSC · 5649-6332 · 684 bp

Back to Sesleria

Pi 0.00293

MarkerSeek score 60.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 2
pi: 0.00293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	755-757 bp	1.0	78.7
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	766-768 bp	1.0	78.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	759-761 bp	1.0	77.6
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	770-772 bp	1.0	77.1
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	45.0	50.0	58.1	57.6	761-763 bp	1.0	75.8