Hotspot

trnK-UUU-rps16

LSC · 3998-4563 · 566 bp

Back to Sesleria

Pi 0.00707
MarkerSeek score 49.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
566
variable_sites
4
pi
0.00707
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TGGACGAATTTGTTGCTCCA AGAAAAAGAAAGAACTTTGAGT 45.0 27.3 58.0 52.1 677 bp 1.0 40.5
trnK-UUU-rps16_p2 TGGACGAATTTGTTGCTCCA AAGAAAAAGAAAGAACTTTGAGT 45.0 26.1 58.0 53.0 678 bp 1.0 40.5
trnK-UUU-rps16_p3 TGGACGAATTTGTTGCTCCA AGAAAAAGAAAGAACTTTGAGTT 45.0 26.1 58.0 53.0 677 bp 1.0 40.5
trnK-UUU-rps16_p4 TGGACGAATTTGTTGCTCCA AAAGAAAAAGAAAGAACTTTGAGT 45.0 25.0 58.0 53.8 679 bp 1.0 40.5
trnK-UUU-rps16_p5 TGGACGAATTTGTTGCTCCA AAGAAAAAGAAAGAACTTTGAGTT 45.0 25.0 58.0 53.8 678 bp 1.0 40.5