Hotspot

ndhF

SSC · 111545-113791 · 2247 bp

Pi 0.011

MarkerSeek score 55.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 116
pi: 0.011
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTCATGAAGGCCTACCGTTG	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.3	58.3	2344-2701 bp	1.0	81.0
ndhF_p2	AATTGGAATTCCTGTCGGGC	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2368-2725 bp	1.0	79.5
ndhF_p3	ATGAAGGCCTACCGTTGGAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.8	58.3	2341-2698 bp	1.0	77.4
ndhF_p4	CTCATGAAGGCCTACCGTTG	TTGAAAGGGGGTAGGATCCG	55.0	55.0	58.3	58.8	2350-2707 bp	1.0	76.7
ndhF_p5	CTCATGAAGGCCTACCGTTG	GGGTAGGATCCGGTAAATCG	55.0	55.0	58.3	57.3	2342-2699 bp	0.941176	74.8