Hotspot

rpl32-trnL-UAG

SSC · 115018-115962 · 945 bp

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Pi 0.0216
MarkerSeek score 83.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
945
variable_sites
94
pi
0.0216
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 924-1109 bp 1.0 90.0
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 925-1110 bp 1.0 90.0
rpl32-trnL-UAG_p3 AGGGATATTGGGCAGCCTTA CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.2 58.1 945-1130 bp 1.0 89.3
rpl32-trnL-UAG_p4 AGGGATATTGGGCAGCCTTA CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.2 58.1 947-1132 bp 1.0 89.3
rpl32-trnL-UAG_p5 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 934-1119 bp 1.0 89.0