Hotspot

ndhF-rpl32

SSC · 113792-114855 · 1064 bp

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Pi 0.0225
MarkerSeek score 68.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1064
variable_sites
107
pi
0.0225
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCACGAATATTGATATGTCTGTTCC GACAACGCTTTTAAGGCTGC 40.0 50.0 57.9 58.3 615-1234 bp 1.0 52.0
ndhF-rpl32_p2 CCACGAATATTGATATGTCTGTTCC TAAGGCTGCCCAATATCCCT 40.0 50.0 57.9 58.2 604-1223 bp 0.941176 49.6
ndhF-rpl32_p3 CCACGAATATTGATATGTCTGTTCC TTAAGGCTGCCCAATATCCC 40.0 50.0 57.9 57.3 605-1224 bp 0.941176 49.6
ndhF-rpl32_p4 TCCACGAATATTGATATGTCTGT TAAGGCTGCCCAATATCCCT 34.8 50.0 55.3 58.2 605-1224 bp 0.941176 49.6
ndhF-rpl32_p5 CCACGAATATTGATATGTCTGTTCC TGCCCAATATCCCTTCCCTT 40.0 50.0 57.9 58.4 598-1217 bp 0.941176 48.1