Hotspot

ycf2-trnL-CAA

IRb · 93105-94348 · 1244 bp

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Pi 0.0798
MarkerSeek score 63.40
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRb
length_bp
1244
variable_sites
244
pi
0.0798
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.36
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 CAGCCCTTTGTCTCTGTGTT AAATGGTAGACACGCGAGAC 50.0 50.0 58.0 58.0 794-1795 bp 1.0 91.7
ycf2-trnL-CAA_p2 CAGCCCTTTGTCTCTGTGTT TGAAATGGTAGACACGCGAG 50.0 50.0 58.0 58.0 796-1797 bp 1.0 91.7
ycf2-trnL-CAA_p3 GGCAAGCGGATCCTTTATGA AAATGGTAGACACGCGAGAC 50.0 50.0 58.0 58.0 971-1972 bp 1.0 91.7
ycf2-trnL-CAA_p4 GTTCTTGCAGAGTGGAACCA AAATGGTAGACACGCGAGAC 50.0 50.0 58.0 58.0 918-1919 bp 1.0 91.7
ycf2-trnL-CAA_p5 GTTCTTGCAGAGTGGAACCA TGAAATGGTAGACACGCGAG 50.0 50.0 58.0 58.0 920-1921 bp 1.0 91.7