Hotspot

psaA-ycf3

LSC · 42662-43370 · 709 bp

Back to Seseli

Pi 0.00819
MarkerSeek score 71.00
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
709
variable_sites
37
pi
0.00819
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT AGCGCAGAATTGGTTGAAGA 50.0 45.0 57.9 58.1 696-857 bp 1.0 86.0
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 683-844 bp 1.0 85.5
psaA-ycf3_p3 TTCTTGAGAAATGACCCGGC AGCGCAGAATTGGTTGAAGA 50.0 45.0 58.2 58.1 719-880 bp 1.0 85.3
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 687-848 bp 1.0 85.3
psaA-ycf3_p5 TTCTTGAGAAATGACCCGGC TTGAAGATCACGAGGCGTTT 50.0 45.0 58.2 57.8 706-867 bp 1.0 84.9