Hotspot

petN-psbM

LSC · 29032-30170 · 1139 bp

Back to Seseli

Pi 0.0123
MarkerSeek score 73.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1139
variable_sites
81
pi
0.0123
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATCC 50.0 50.0 58.2 57.9 881-1348 bp 0.941176 83.3
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATCC 50.0 50.0 57.6 57.9 820-1287 bp 0.941176 81.9
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT GCTACTGCACTGTTCATCCT 50.0 50.0 58.2 57.6 880-1347 bp 0.941176 81.0
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT GCTACTGCACTGTTCATCCT 50.0 50.0 57.6 57.6 819-1286 bp 0.941176 79.6
petN-psbM_p5 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATCC 50.0 50.0 57.1 57.9 882-1349 bp 0.941176 77.4