Hotspot

trnC-GCA-petN

LSC · 28248-28941 · 694 bp

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Pi 0.0117
MarkerSeek score 75.90
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
694
variable_sites
48
pi
0.0117
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 712-819 bp 1.0 87.1
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 773-880 bp 1.0 85.2
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 730-837 bp 1.0 84.8
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 791-898 bp 1.0 82.9
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG GCCCAAGCGAGACTTACTAT 50.0 50.0 58.1 57.1 711-818 bp 1.0 81.2