Hotspot

rpoB-trnC-GCA

LSC · 26967-28176 · 1210 bp

Back to Seseli

Pi 0.015
MarkerSeek score 69.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1210
variable_sites
101
pi
0.015
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCCTTCATTTACATCTCGGAGC CCGGATTTGAACTGGGGAAA 45.5 50.0 57.9 58.1 1138-1384 bp 0.941176 68.4
rpoB-trnC-GCA_p2 CCTTCATTTACATCTCGGAGCA CCGGATTTGAACTGGGGAAA 45.5 50.0 58.2 58.1 1137-1383 bp 0.941176 67.2
rpoB-trnC-GCA_p3 TCCTTCATTTACATCTCGGAGC TTATTGATCAGGCGACACCC 45.5 50.0 57.9 57.7 1156-1402 bp 0.941176 66.4
rpoB-trnC-GCA_p4 TCCTTCATTTACATCTCGGAGC AAAAGGATTTGCAGTCCCCC 45.5 50.0 57.9 58.4 1120-1366 bp 0.941176 66.2
rpoB-trnC-GCA_p5 GCCAGGTATTGTAGACATTCCT CCGGATTTGAACTGGGGAAA 45.5 50.0 57.6 58.1 1156-1402 bp 0.941176 65.5