Hotspot

rps2-rpoC2

LSC · 16342-16552 · 211 bp

Back to Seseli

Pi 0.0252
MarkerSeek score 79.30
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
211
variable_sites
22
pi
0.0252
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 AATGAACTCCGGCCTTCATC TGCCTTTCAAAGAATTTCCACG 50.0 40.9 58.0 58.0 335-359 bp 0.941176 66.3
rps2-rpoC2_p2 CGGCCTTCATCATCTCTTCC TGCCTTTCAAAGAATTTCCACG 55.0 40.9 58.1 58.0 326-350 bp 0.941176 65.8
rps2-rpoC2_p3 CCGGCCTTCATCATCTCTTC TGCCTTTCAAAGAATTTCCACG 55.0 40.9 58.1 58.0 327-351 bp 0.941176 65.7
rps2-rpoC2_p4 AATGAACTCCGGCCTTCATC GCCTTTCAAAGAATTTCCACGA 50.0 40.9 58.0 57.8 334-358 bp 0.941176 64.6
rps2-rpoC2_p5 AAAATGAACTCCGGCCTTCA TGCCTTTCAAAGAATTTCCACG 45.0 40.9 57.7 58.0 337-361 bp 0.882353 61.7