Hotspot

psbA-trnK-UUU

LSC · 1372-1572 · 201 bp

Back to Seseli

Pi 0.0238
MarkerSeek score 72.70
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
201
variable_sites
25
pi
0.0238
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TAAAGACGGTTTTCGGTGCT CTGAAAAATGCAAGCACGGT 45.0 45.0 57.8 57.9 383-403 bp 1.0 84.8
psbA-trnK-UUU_p2 TAAAGACGGTTTTCGGTGCT ACTGAAAAATGCAAGCACGG 45.0 45.0 57.8 57.9 384-404 bp 1.0 84.7
psbA-trnK-UUU_p3 GTAAAGACGGTTTTCGGTGC CTGAAAAATGCAAGCACGGT 50.0 45.0 57.7 57.9 384-404 bp 1.0 84.2
psbA-trnK-UUU_p4 TAAAGACGGTTTTCGGTGCT TTGGGGAGGGGTGTTTATCT 45.0 50.0 57.8 57.9 363-375 bp 0.941176 79.5
psbA-trnK-UUU_p5 GTAAAGACGGTTTTCGGTGC TTGGGGAGGGGTGTTTATCT 50.0 50.0 57.7 57.9 364-376 bp 0.941176 79.0