Hotspot

trnH-GUG-psbA

LSC · 115-309 · 195 bp

Back to Seseli

Pi 0.0683
MarkerSeek score 82.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
195
variable_sites
59
pi
0.0683
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 307-348 bp 1.0 90.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 321-362 bp 1.0 90.7
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 320-361 bp 1.0 90.7
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 322-363 bp 1.0 90.7
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 257-298 bp 1.0 90.4