Hotspot

rpl20

LSC · 69823-70209 · 387 bp

Back to Sesamum

Pi 0.0103

MarkerSeek score 53.60

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 387
variable_sites: 4
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	GAGTTCATTCTCCGGGGAAC	TAATAGACCAAGGCGGGGAA	55.0	50.0	58.0	58.1	489 bp	1.0	79.5
rpl20_p2	GAGTTCATTCTCCGGGGAAC	TTAATAGACCAAGGCGGGGA	55.0	50.0	58.0	58.1	490 bp	1.0	79.5
rpl20_p3	GTTCATTCTCCGGGGAACTC	TAATAGACCAAGGCGGGGAA	55.0	50.0	58.0	58.1	487 bp	1.0	79.5
rpl20_p4	GTTCATTCTCCGGGGAACTC	TTAATAGACCAAGGCGGGGA	55.0	50.0	58.0	58.1	488 bp	1.0	79.5
rpl20_p5	GTTCATTCTCCGGGGAACTC	GACCAAGGCGGGGAAAATAA	55.0	50.0	58.0	58.2	482 bp	1.0	79.2