Hotspot

ndhD-psaC

SSC · 117119-117242 · 124 bp

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Pi 0.0726

MarkerSeek score 72.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 124
variable_sites: 9
pi: 0.0726
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAGCAAGGAAAATGATTCGT	ACTGTGTTGGTTGTAAGCGA	35.0	45.0	53.8	57.7	251 bp	1.0	46.4
ndhD-psaC_p2	TGTTAAGCAAGGAAAATGATTCGT	ACTGTGTTGGTTGTAAGCGA	33.3	45.0	57.7	57.7	255 bp	1.0	45.7
ndhD-psaC_p3	AAGCAAGGAAAATGATTCGT	GCCTGTCCAACGGATTTCTT	35.0	50.0	53.8	58.5	222 bp	1.0	45.6
ndhD-psaC_p4	TGTTAAGCAAGGAAAATGATTCGT	GCCTGTCCAACGGATTTCTT	33.3	50.0	57.7	58.5	226 bp	1.0	44.9
ndhD-psaC_p5	AAGCAAGGAAAATGATTCGT	CGAGGACTGTGTTGGTTGTA	35.0	50.0	53.8	57.5	256 bp	1.0	44.8