Hotspot

rpl32-tRNA-Leu (UAG)

SSC · 113332-114214 · 883 bp

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Pi 0.0114
MarkerSeek score 52.20
Primer available yes
Label rpl32-tRNA-Leu (UAG)

Feature Metadata

label_name
rpl32-tRNA-Leu (UAG)
feature_type
igs
region
SSC
length_bp
883
variable_sites
10
pi
0.0114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-tRNA-Leu (UAG)_p1 TCACCGGACCTTACCTCTTT GGTAAAAATCGCACTTGGGC 50.0 50.0 58.0 58.0 2023-2032 bp 1.0 81.3
rpl32-tRNA-Leu (UAG)_p2 TCACCGGACCTTACCTCTTT GTAAAAATCGCACTTGGGCC 50.0 50.0 58.0 58.0 2022-2031 bp 1.0 81.3
rpl32-tRNA-Leu (UAG)_p3 TTCACCGGACCTTACCTCTT GGTAAAAATCGCACTTGGGC 50.0 50.0 58.0 58.0 2024-2033 bp 1.0 81.3
rpl32-tRNA-Leu (UAG)_p4 TTCACCGGACCTTACCTCTT GTAAAAATCGCACTTGGGCC 50.0 50.0 58.0 58.0 2023-2032 bp 1.0 81.3
rpl32-tRNA-Leu (UAG)_p5 TCACCGGACCTTACCTCTTT TTTAGGAGATGACATGCCGC 50.0 50.0 58.0 58.0 1621-1628 bp 1.0 81.2