Hotspot

psbH-petB

LSC · 75968-76091 · 124 bp

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Pi 0.0323

MarkerSeek score 71.00

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 4
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTCGCAATGGCTCTATTTGC	TCTCTATCGAGGTGACAGACC	50.0	52.4	57.9	58.1	284 bp	1.0	71.6
psbH-petB_p2	CTTTGATGGGTGTCGCAATG	TCTCTATCGAGGTGACAGACC	50.0	52.4	57.7	58.1	295 bp	1.0	70.3
psbH-petB_p3	GGTGTCGCAATGGCTCTATT	TCTCTATCGAGGTGACAGACC	50.0	52.4	58.3	58.1	287 bp	1.0	69.8
psbH-petB_p4	GTCGCAATGGCTCTATTTGC	CTCTATCGAGGTGACAGACC	50.0	55.0	57.9	56.6	283 bp	1.0	68.8
psbH-petB_p5	GTCGCAATGGCTCTATTTGC	GTTCGTGTTCCAGATACTCGA	50.0	47.6	57.9	57.5	330 bp	1.0	68.2