Hotspot

petA-psbJ

LSC · 64384-65387 · 1004 bp

Back to Sesamum

Pi 0.00801

MarkerSeek score 63.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1004
variable_sites: 8
pi: 0.00801
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	1152-1157 bp	1.0	65.1
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	1154-1159 bp	1.0	65.1
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	GCCGATACTACTGGAAGGAT	45.0	50.0	57.7	56.2	1151-1156 bp	1.0	64.3
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	GCCGATACTACTGGAAGGAT	45.0	50.0	57.6	56.2	1231-1236 bp	1.0	63.2
petA-psbJ_p5	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGATT	50.0	47.6	58.2	57.0	1152-1157 bp	1.0	63.2