Hotspot

tRNA-Phe (GAA)-ndhJ

LSC · 48960-49630 · 671 bp

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Pi 0.00765

MarkerSeek score 65.60

Primer available yes

Label tRNA-Phe (GAA)-ndhJ

Feature Metadata

label_name: tRNA-Phe (GAA)-ndhJ
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 5
pi: 0.00765
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Phe (GAA)-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	796-813 bp	1.0	81.5
tRNA-Phe (GAA)-ndhJ_p2	GGATGCTACATTGGGACTGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	818-835 bp	1.0	81.2
tRNA-Phe (GAA)-ndhJ_p3	GGGATGCTACATTGGGACTG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	819-836 bp	1.0	81.2
tRNA-Phe (GAA)-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	760-777 bp	1.0	81.2
tRNA-Phe (GAA)-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	784-801 bp	1.0	80.4