Hotspot

psaA-ycf3

LSC · 42331-43072 · 742 bp

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Pi 0.00282

MarkerSeek score 64.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 2
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	919-939 bp	1.0	82.0
psaA-ycf3_p2	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	901-921 bp	1.0	82.0
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	848-868 bp	1.0	81.3
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	852-872 bp	1.0	81.0
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	915-935 bp	1.0	80.3