Hotspot

rps2-rpoC2

LSC · 16664-16870 · 207 bp

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Pi 0.0194

MarkerSeek score 64.10

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 207
variable_sites: 4
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTCCAGCATCTCTTCCAAG	TATTCGAGGGGGAAACGAGA	55.0	50.0	57.7	57.9	371-372 bp	1.0	77.3
rps2-rpoC2_p2	CCTCCAGCATCTCTTCCAAG	CGAGGGGGAAACGAGAGATA	55.0	55.0	57.7	58.3	367-368 bp	1.0	76.0
rps2-rpoC2_p3	AAATGAACTCCTGCCTCCAG	TATTCGAGGGGGAAACGAGA	50.0	50.0	57.5	57.9	384-385 bp	1.0	75.8
rps2-rpoC2_p4	AAATGAACTCCTGCCTCCAG	CGAGGGGGAAACGAGAGATA	50.0	55.0	57.5	58.3	380-381 bp	1.0	74.5
rps2-rpoC2_p5	CCATGGCCAAAATGAACTCC	TATTCGAGGGGGAAACGAGA	50.0	50.0	57.3	57.9	393-394 bp	1.0	74.4