Hotspot

tRNA-His (GUG)-psbA

LSC · 91-382 · 292 bp

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Pi 0.0205

MarkerSeek score 64.30

Primer available yes

Label tRNA-His (GUG)-psbA

Feature Metadata

label_name: tRNA-His (GUG)-psbA
feature_type: igs
region: LSC
length_bp: 292
variable_sites: 6
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-His (GUG)-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	359-363 bp	1.0	79.7
tRNA-His (GUG)-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	373-377 bp	1.0	79.6
tRNA-His (GUG)-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	372-376 bp	1.0	79.6
tRNA-His (GUG)-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	374-378 bp	1.0	79.6
tRNA-His (GUG)-psbA_p5	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	411-415 bp	1.0	78.7