Hotspot

ndhF-trnL-UAG

SSC · 117160-118601 · 1442 bp

Back to Senna

Pi 0.0436
MarkerSeek score 78.00
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1442
variable_sites
175
pi
0.0436
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 TCCATGAATATTGATGTGTATATTCCA AGCGTGTCTACCGATTTCAC 29.6 50.0 56.7 58.0 1499-1554 bp 1.0 52.0
ndhF-trnL-UAG_p2 TCCATGAATATTGATGTGTATATTCCA CAGCGTGTCTACCGATTTCA 29.6 50.0 56.7 58.0 1500-1555 bp 1.0 52.0
ndhF-trnL-UAG_p3 TCCATGAATATTGATGTGTATATTCCA TCCTAAGAGCAGCGTGTCTA 29.6 50.0 56.7 57.9 1509-1564 bp 1.0 52.0
ndhF-trnL-UAG_p4 TCCATGAATATTGATGTGTATATTCCA CCTAAGAGCAGCGTGTCTAC 29.6 55.0 56.7 57.8 1508-1563 bp 1.0 52.0
ndhF-trnL-UAG_p5 TCCATGAATATTGATGTGTATATTCCA CTAAGAGCAGCGTGTCTACC 29.6 55.0 56.7 57.8 1507-1562 bp 1.0 52.0