Hotspot

rps11-rpl36

LSC · 83514-83790 · 277 bp

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Pi 0.0834
MarkerSeek score 73.30
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
277
variable_sites
52
pi
0.0834
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.93
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 CGTCCATTTCTACGTGAACCA GGCGCGGACGAATTATAGTA 47.6 50.0 58.3 57.7 347-436 bp 1.0 78.7
rps11-rpl36_p2 CGTCCATTTCTACGTGAACCA GTAGGCGCGGACGAATTATA 47.6 50.0 58.3 57.7 350-439 bp 1.0 78.7
rps11-rpl36_p3 CGTCCATTTCTACGTGAACC GGCGCGGACGAATTATAGTA 50.0 50.0 56.6 57.7 347-436 bp 1.0 77.8
rps11-rpl36_p4 CGTCCATTTCTACGTGAACC GTAGGCGCGGACGAATTATA 50.0 50.0 56.6 57.7 350-439 bp 1.0 77.8
rps11-rpl36_p5 CGTCCATTTCTACGTGAACCA TAGGCGCGGACGAATTATAG 47.6 50.0 58.3 57.4 349-438 bp 1.0 76.4