Hotspot

petA-psbJ

LSC · 66425-67618 · 1194 bp

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Pi 0.0427
MarkerSeek score 72.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1194
variable_sites
134
pi
0.0427
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.96
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TCAAAGCTACCGTCCCACTA AACTGGCCGTTTTGATCCTT 50.0 45.0 58.1 58.0 2108-2403 bp 0.846154 82.5
petA-psbJ_p2 TCAAAGCTACCGTCCCACTA ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.1 58.0 2107-2402 bp 0.846154 82.5
petA-psbJ_p3 GCTCCCTCCGATCGTATTTC AACTGGCCGTTTTGATCCTT 55.0 45.0 57.9 58.0 2374-2435 bp 0.307692 54.6
petA-psbJ_p4 GCTCCCTCCGATCGTATTTC ACTGGCCGTTTTGATCCTTT 55.0 45.0 57.9 58.0 2373-2434 bp 0.307692 54.6
petA-psbJ_p5 GCTCCCTCCGATCGTATTTC TGCGGGTTGGTTATTTGTCA 55.0 45.0 57.9 58.0 2476-2537 bp 0.307692 54.3