Hotspot

ndhC-trnV-UAC

LSC · 54914-55480 · 567 bp

Back to Senna

Pi 0.0777
MarkerSeek score 75.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
567
variable_sites
114
pi
0.0777
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGAGAAGCGGCCATAAACTG AAAAGATGCACGTTGGGTCT 50.0 45.0 58.0 58.0 1186-1426 bp 1.0 91.5
ndhC-trnV-UAC_p2 AGAGAAGCGGCCATAAACTG TGCACGTTGGGTCTGTAAAA 50.0 45.0 58.0 58.0 1180-1420 bp 1.0 91.3
ndhC-trnV-UAC_p3 TTGTTACTGTGCCTGCTGTT AAAAGATGCACGTTGGGTCT 45.0 45.0 57.9 58.0 1318-1558 bp 1.0 90.7
ndhC-trnV-UAC_p4 TTGTTACTGTGCCTGCTGTT TGCACGTTGGGTCTGTAAAA 45.0 45.0 57.9 58.0 1312-1552 bp 1.0 90.5
ndhC-trnV-UAC_p5 TGGTTCAGGCATTTGCTCAT AAAAGATGCACGTTGGGTCT 45.0 45.0 58.1 58.0 1368-1608 bp 1.0 90.2