Hotspot

psbZ-trnG-GCC

LSC · 39828-40428 · 601 bp

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Pi 0.0866
MarkerSeek score 78.60
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
601
variable_sites
141
pi
0.0866
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.94
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.0 57.7 719-1171 bp 1.0 89.3
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 722-1174 bp 1.0 87.8
psbZ-trnG-GCC_p3 TCCTGATGGTTGGTCGAGTA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 57.8 57.7 715-1167 bp 1.0 87.6
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC GCGTCTTCTCTTTGGCAAAG 55.0 50.0 58.3 57.7 720-1172 bp 1.0 87.4
psbZ-trnG-GCC_p5 TTCTCCTGATGGTTGGTCGA GCGTCTTCTCTTTGGCAAAG 50.0 50.0 58.4 57.7 718-1170 bp 1.0 86.5