Hotspot

trnC-GCA-petN

LSC · 30296-31330 · 1035 bp

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Pi 0.0353
MarkerSeek score 74.30
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1035
variable_sites
110
pi
0.0353
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 1038-1141 bp 1.0 90.1
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG ACTTCTGCCCCATACTACGA 50.0 50.0 58.1 57.8 1092-1195 bp 1.0 89.9
trnC-GCA-petN_p3 GTATCATTTTGGCGGCATGG AGCCCAAGCGAGACTTACTA 50.0 50.0 57.9 58.2 1090-1193 bp 1.0 89.5
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG CACTTCTGCCCCATACTACG 50.0 55.0 58.1 57.8 1093-1196 bp 1.0 89.3
trnC-GCA-petN_p5 GTATCATTTTGGCGGCATGG ACTTCTGCCCCATACTACGA 50.0 50.0 57.9 57.8 1144-1247 bp 1.0 89.1