Hotspot

atpF-atpH

LSC · 14311-15077 · 767 bp

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Pi 0.0811
MarkerSeek score 66.30
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
767
variable_sites
144
pi
0.0811
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.59
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CTACGCTGCGTCTAGACTTC ATTTTGGGTGCGAGGAACTT 55.0 45.0 57.9 58.0 1077-1532 bp 1.0 91.2
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG ATTTTGGGTGCGAGGAACTT 50.0 45.0 57.9 58.0 777-1207 bp 1.0 91.0
atpF-atpH_p3 CTACGCTGCGTCTAGACTTC ATTTCTGCCGCTTCCGTTAT 55.0 45.0 57.9 58.0 1042-1497 bp 1.0 90.9
atpF-atpH_p4 ATTAAACCCGAAACTCCCGG ATTTCTGCCGCTTCCGTTAT 50.0 45.0 57.9 58.0 742-1172 bp 1.0 90.7
atpF-atpH_p5 GGGATCATGCACCTTTTCCT ATTTTGGGTGCGAGGAACTT 50.0 45.0 57.9 58.0 939-1368 bp 0.923077 87.7