Hotspot

trnR-UCU-atpA

LSC · 11097-11437 · 341 bp

Back to Senna

Pi 0.0807
MarkerSeek score 74.50
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
341
variable_sites
75
pi
0.0807
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC TCAGGAACAGATGGAACTCT 47.6 45.0 57.3 55.5 409-750 bp 1.0 58.2
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC TCAGGAACAGATGGAACTCTT 47.6 42.9 57.3 56.3 409-750 bp 1.0 56.7
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC TTCAGGAACAGATGGAACTCT 47.6 42.9 57.3 56.3 410-751 bp 1.0 56.7
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC AGCTATTCAGGAACAGATGGA 47.6 42.9 57.3 56.1 415-756 bp 1.0 55.5
trnR-UCU-atpA_p5 TGGATAGGACAGAGGTCTTCT TCAGGAACAGATGGAACTCT 47.6 45.0 56.9 55.5 444-785 bp 1.0 55.2